Neurofilament protein phosphorylation. Species generality and reaction characteristics.
نویسندگان
چکیده
منابع مشابه
Phosphorylation-induced mechanical regulation of intrinsically disordered neurofilament protein assemblies
The biological function of protein assemblies was conventionally equated with a unique three-dimensional protein structure and protein-specific interactions. However, in the past 20 years it was found that some assemblies contain long flexible regions that adopt multiple structural conformations. These include neurofilament (NF) proteins that constitute the stressresponsive supportive network o...
متن کاملMultiple phosphorylation sites in mammalian neurofilament polypeptides.
The 68,000, 145,000, and 200,000 dalton subunits of neurofilaments from rat spinal cord and brain stem were shown by chemical phosphate analysis to contain about 3, 9, and 22 mol of phosphate/mol of polypeptide, respectively. All three subunits contained phosphoserine while the 145,000 dalton polypeptide also contained phosphothreonine. The high phosphate content of the 200,000 dalton polypepti...
متن کاملAberrant neurofilament phosphorylation in Alzheimer disease.
Alzheimer tangles, despite their location in neuronal perikarya, react immunocytochemically with monoclonal antibodies to phosphorylated epitopes of neurofilaments. Normal perikarya do not contain phosphorylated neurofilaments. The aberrant phosphorylation in both plaques and tangles seems to be largely restricted to individual phosphorylation sites among the many sites available in neurofilame...
متن کاملProperties of neurofilament protein kinase.
Neurofilament (NF) protein kinase, partially purified from NF preparations [Toru-Delbauffe & Pierre (1983) FEBS Lett. 162, 230-234], was found to be distinct from both the casein kinase present in NFs and the cyclic AMP-dependent protein kinase which is able to phosphorylate NFs. NF-kinase phosphorylated the three NF protein components. The amount of phosphate incorporated per molecule was high...
متن کاملDifferential dynamics of neurofilament-H protein and neurofilament-L protein in neurons
Neurofilaments (NFs) are composed of triplet proteins, NF-H, NF-M, and NF-L. To understand the dynamics of NFs in vivo, we studied the dynamics of NF-H and compared them to those of NF-L, using the combination of microinjection technique and fluorescence recovery after photobleaching. In the case of NF-L protein, the bleached zone gradually restored its fluorescence intensity with a recovery ha...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34595-2